A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint

A non-canonical Aux/IAA gene MsIAA32 regulates peltate glandular trichome development in spearmint

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Phytohormone auxin controls various aspects of plant growth and development.The typical auxin signalling involves the degradation of canonical Aux/IAA proteins upon auxin perception releasing the auxin response iphone 14 price texas factors (ARF) to activate auxin-regulated gene expression.Extensive research has been pursued in deciphering the role of canonical Aux/IAAs, however, the function of non-canonical Aux/IAA genes remains elusive.Here we identified a non-canonical Aux/IAA gene, MsIAA32 from spearmint (Mentha spicata), which lacks the TIR1-binding domain and shows its involvement in the development of peltate glandular trichomes (PGT), which are the sites for production and storage of commercially important essential oils.

Using yeast two-hybrid studies, two canonical Aux/IAAs, MsIAA3, MsIAA4 and an harry potter magsafe case ARF, MsARF3 were identified as the preferred binding partners of MsIAA32.Expression of a R2R3-MYB gene MsMYB36 and a cyclin gene MsCycB2-4 was altered in MsIAA32 suppressed plants indicating that these genes are possible downstream targets of MsIAA32 mediated signalling.Ectopic expression of MsIAA32 in Arabidopsis affected non-glandular trichome formation along with other auxin related developmental traits.Our findings establish the role of non-canonical Aux/IAA mediated auxin signalling in PGT development and reveal species-specific functionalization of Aux/IAAs.